To reveal the underlying mechanisms of the proliferation of cellular organelles


  To accomplish the challenging aims of our research projects, we use the primitive unicellular alga Cyanidioschyzon merolae (Matsuzaki et al. 2004). This single-celled organism contains only a few membranous organelles: one cell nucleus, one mitochondrion, one plastid , one peroxisome, a simple-shaped ER, one Golgi with two cisternae, and a few vacuoles. This alga has only 4,775 genes in its genome. A synchronous, drug-free cultivation method has been established for C. merolae, and the absence of a rigid cell wall has made it possible to develop effective organelle isolation methods. Many genetic techniques, as well as genome- and system-wide multi-omics analyses, can now be carried out using this system. Importantly, this alga is the only organism for which methods have been established for the isolation of intact plastid- and mitochondrial-division machinery (Yoshida et al. 2006; 2009; 2010; 2017). By using the simplest eukaryote, combined with genomcis, transomics, bioinfomatics, single-molecule imaging, live-imaging, genome editing, and emerging biotechnologies, we are challenging to reveal the underlying mechanisms of the proliferation of cellular organelles.


© 2019 by Yamato Yoshida